Secondary treatment or Biological  treatment:
              
                - The primary effluent is passed into large  aeration tanks.
- This allows vigorous growth of useful aerobic  microbes into flocs.
- The growth of microbes consumes the major part  of the organic matter in the effluent. This significantly reduces the BOD (biochemical oxygen demand) of the  effluent.
- BOD refers to the amount of oxygen required to  oxidize total organic matter by bacteria, present in one liter of water.
- BOD is the measures of the organic matter  present in the water.
- Greater  the BOD of the waste water more is its polluting potential.
- Once the BOD of sewage reduced significantly,  the effluent is then passed into the settling tank where the bacterial ’flocs’  are allowed to sediment. This sediment is called activated sludge.
- Small part of activated sludge is pumped back to  aeration tank to serve as the inoculums.
- The remaining sludge is pumped into anaerobic sludge digester.
- In the anaerobic sludge digester there is other  kinds of bacteria which grow anaerobically, digest the bacteria and fungi in  the sludge.
- During this digestion bacteria produce biogas, (mixture of methane, hydrogen  sulphide and carbon dioxide)
- The effluent from the secondary treatment plant  is released into natural water body like rivers and streams.
- Ganga  Action Plan and Yamuna Action Plan initiated by Ministry of Environment and Forest to save these major rivers of  our country.
- It is proposed to build a large number of sewage  treatment plants so that only treated sewage may be discharged into the rivers.
MICROBES IN PRODUCTION OF BIOGAS:
              
                - Biogas is a mixture of gases (predominantly methane)  produced by the microbial activity and is used as fuel.
- Certain bacteria grow anaerobically on  cellulosic material, produce large amount of methane along with CO2  and H2S. These bacteria are collectively called methanogens. One common bacterium is Methanobacterium.
- These bacteria present in the rumen of cattle,  plays essential role in nutrition of cattle by digesting cellulose. Hence the  excreta (dung) used for the production of biogas.
MICROBES AS BIOCONTROL AGENT:
              
                - Biocontrol refers to the use of biological  methods for controlling plant diseases and pests.
- Effect of use of chemical, insecticide and  pesticide to control disease and pests:
                  
                    - These chemicals are toxic and extremely harmful  to human beings and animals
- Polluting our environment (soil, ground water),  fruits, and vegetables. 
- Soil is polluted through use of weedicides to  remove weeds.
 
Biological control of pest and disease:
              
                - Use of biocontrol measures will greatly reduce  our dependence on toxic chemical and pesticides.
- The Ladybird and Dragonflies are used to get rid  of aphids and mosquitoes.
- Bacillus thuringiensis (Bt) used to  control butterfly caterpillars.
- Dried spores are mixed with water and sprayed  onto vulnerable plants, where these are eaten by the insect larvae.
- In the gut of the larvae, the toxin is released  and the larvae get killed.
- Trichodermaa free living fungus  used to control several plant pathogens.
- Baculoviruses are pathogen that attack insects  and other arthropods
- The majority of baculoviruses used as biological control agents are in the genus Nucleopolyhedrovirus.
- These viruses are excellent candidates for  species-specific, narrow spectrum insecticidal application.
- They have no negative impacts on plants,  mammals, birds, fish, etc.
- This is very use full in integrated pest  managements (IPM).
MICROBES AS BIOFERTILIZERS:
              
                - Biofertilizers are organisms that enrich the nutrient quality of the soil.
- Main biofertilizers are the bacteria, fungi and  cyanobacteria.
- Rhizobiumform root nodules in  legumes and fix atmospheric nitrogen.
- Azospiriliumand Azotobacter free living bacteria fix atmospheric nitrogen and thus increasing  nitrogen content of the soil.
- Mycorrhiza: fungi symbiotically associated with root of plants.
                - Many members of the genus Glomus form Mycorrhiza.
                  
                    - Provide phosphorus to the plants from the soil.
- Make the plant resistant to root-borne pathogen.
- Increase tolerance to salinity and drought.
 
 
- Cyanobacteria like Anabaena, Nostoc, and Oscillatoria etc:
                  
                    - Fix atmospheric nitrogen.
- add organic matter to the soil and 
- Increase soil fertility.
 
                      - DO :         Dissolved  Oxygen
- GAP :       Ganga  Action  Plan
- KVIC :      Khadi  and  Village   Industries  Commission
- TMV :       Tobacco  Mosaic  Virus
- YAP :        Yamuna  Action  Plan
- IPM:         Integrated Pest Management.